Evidence profile
NAD+
NAD+ study rows link each listed finding to its source, model or population, and endpoint.
Cited headline finding
The study characterized NAD dependence in a yeast Sir2 system. ↗
Yeast Sir2 systemImai et al., Nature 2000In vitro
Mechanism scope
How cited research characterizes NAD+
The cited NAD+ studies characterize substrate dependence in yeast and mouse Sir2 systems and cleavage and consumption in PARP biochemical systems.
Sources: Imai et al., Nature 2000 ↗ · Smith et al., PNAS 2001 ↗ · Kleine et al., Journal of Biological Chemistry 2008 ↗
Open question
What the cited NAD+ sources do not resolve
The cited NAD+ studies do not establish findings beyond their yeast, mouse, and biochemical systems.
Sources: Imai et al., Nature 2000 ↗ · Smith et al., PNAS 2001 ↗ · Kleine et al., Journal of Biological Chemistry 2008 ↗
| Finding | Year | n | Model or population | Endpoint | Source | Evidence tier |
|---|---|---|---|---|---|---|
| The biochemical study characterized NAD cleavage and consumption by PARP systems. | 2008 | Not stated | PARP biochemical systems | NAD cleavage and consumption | Kleine et al., Journal of Biological Chemistry 2008 ↗ | In vitro |
| The study characterized NAD dependence in mouse Sir2 systems. | 2001 | Not stated | Mouse Sir2 systems | Enzyme substrate dependence | Smith et al., PNAS 2001 ↗ | Animal |
| The study characterized NAD dependence in a yeast Sir2 system. | 2000 | Not stated | Yeast Sir2 system | Enzyme substrate dependence | Imai et al., Nature 2000 ↗ | In vitro |
Regulatory status
NAD+ appears in Health Canada's peptide-drug advisory. ↗
Reviewer: Ember Peptides source-verification editorial review · Review date: 2026-09-13